Donatello: Mitchell Whitfield Leonardo: James Arnold Taylor Michaelangelo: Mikey Kelley Raphael: Nolan North Splinter: Mako April O'Neil: Sarah Michelle Gellar Casey Jones: Chris Evans Karai: Ziyi Zhang Max Winters: Patrick Stewart General Gato: Fred Tatasciore Colonel Santino: John Di Maggio General Serpiente: Paula Mattioli General Aquila: Kevin Michael Richardson © Warner Bros.
Donatello: Richard Yee Leonardo: Gabe Khouth Michaelangelo: Jarred Blancard Raphael: Mitchell A. Lee Yuen Splinter: Fiona Scott Venus: Nicole Parker Bonesteel: Scott McNeil Chi Chu: Lauren Attadia Bing: Colin Musback © Fox Television
Donatello: Jim Raposa Leonardo: Mark Caso Michaelangelo: David Fraser Raphael: Matt Hill Splinter: Kevin Clash April O'Neil: Paige Turco Casey Jones: Elias Koteas Lord Norinaga: Sab Shimono Walker: Stuart Wilson © New Line Cinema
Donatello: Leif Tilden Leonardo: Mark Caso Michaelangelo: Michelan Sisti Raphael: Kenn Scott Splinter: Kevin Clash April O'Neil: Paige Turco Shredder: Francois Chau Super Shredder: Kevin Nash Prof. Jordan Perry: David Warner © New Line Cinema
Donatello: Leif Tilden Leonardo: David Forman Michaelangelo: Michelan Sisti Raphael: Josh Pais Splinter: Kevin Clash Shredder: James Saito April O'Neil: Judith Hoag Casey Jones: Elias Koteas Danny Pennington: Michael Turney © New Line Cinema
