Spider-Man: Tobey Maguire
Mary Jane Watson: Kirsten Dunst
Harry Osborn: James Franco
Sandman: Thomas Haden Church
Venom: Topher Grace
May Parker: Rosemary Harris
J. Jonah Jameson: J.K. Simmons
Gwen Stacy: Bryce Dallas Howard
© Columbia Pictures

Spider-Man: Tobey Maguire
Mary Jane Watson: Kirsten Dunst
Harry Osborn: James Franco
Dr. Octopus: Alfred Molina
May Parker: Rosemary Harris
J. Jonah Jameson: J.K. Simmons
Dr. Curt Connors: Dylan Baker
John Jameson: Daniel Gillies
© Columbia Pictures

Spider-Man: Tobey Maguire
Mary Jane Watson: Kirsten Dunst
Harry Osborn: James Franco
Green Goblin: Willem Dafoe
May Parker: Rosemary Harris
Ben Parker: Cliff Robertson
J. Jonah Jameson: J.K. Simmons
Flash Thompson: Joe Manganiello
© Columbia Pictures

Spider-Man: Dan Poole
Green Goblin: Jim Kinstle
Harry Osborn: Bob Tull
© Alpha Dog Productions

Spider-Man: Kousuke Kayama
Prof. Monster: Mitsuo Ando
Amazonesu: Yukie Kagawa
Machine Bem: Shozo Izuka
© Toei Animation

Spider-Man: Nicholas Hammond
J. Jonah Jameson: Robert F. Simon
Julie Masters: Ellen Bry
Rita Conway: Chip Fields
May Parker: Irene Tedrow
© Charles Fries Productions

Spider-Man: Nicholas Hammond
J. Jonah Jameson: David White
Capt. Barbera: Michael Pataki
Judy Tyler: Lisa Eilbacher
May Parker: Irene Tedrow
© Charles Fries Productions

Spider-Man: Danny Seagren
Main Cast: Various
Short Circus: Various
© Children's Television Workshop